Dunce mutants of Drosophila melanogaster: mutants defective in the cyclic AMP phosphodiesterase enzyme system
نویسندگان
چکیده
The cyclic AMP and cyclic GMP phosphodiesterase activities present in flies of six mutant strains of the dunce gene and in the parent wild-type strains are characterized. All of the mutants exhibit aberrant cyclic AMP metabolism. The mutant strains dunceM14, dunceM11, and dunceML appear to be amorphic, because they completely lack the cAMP-specific phosphodiesterase normally present in adult flies. These strains exhibit extremely high levels of cAMP. The mutant strains dunce1, dunce2, and dunceCK are hypomorphic and exhibit reduced levels of the cAMP-specific phosphodiesterase. These strains exhibit less marked increases in cAMP content compared with the three amorphic strains. The dunce2 strain possesses a residual enzyme activity that exhibits anomalous kinetics compared with those of the normal enzyme. The possibility that the dunce locus is the structural gene for the cAMP-specific phosphodiesterase is discussed.
منابع مشابه
The Dunce gene of Drosophila: roles of Ca2+ and calmodulin in adenosine 3':5'-cyclic monophosphate-specific phosphodiesterase activity.
Two genetically distinct forms of cyclic nucleotide phosphodiesterases are present in adult Drosophila melanogaster. Form II, which specifically hydrolyzes adenosine 3':5'-cyclic monophosphate (cAMP), is controlled by the dunce+ gene. Mutants of this gene either eliminate this enzyme form entirely or alter its kinetic and thermal properties, suggesting that dunce+ is the structural gene for thi...
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ورودعنوان ژورنال:
- The Journal of Cell Biology
دوره 90 شماره
صفحات -
تاریخ انتشار 1981